Garlic extracts reported activities include reducing cholesterol, tumor suppressing, and acting as an antifungal, antibacterial, antiviral and antiparasitic agent reuter et al. Antimicrobial and antioxidant activity of crude onion allium cepa, l. Antioxidant properties, antimicrobial activity, natural additive, garlic peel extract 1. Antimicrobial activity of extracts of red mangle bark, garlic clove, onion and lemon peel author. In this paper we evaluated the antimicrobial activity of crude extracts of garlic against seventeen bacterial pathogens and also an attempt has been made to study the effect of temperature and ph on the stability of the extracts against two gram positive and two gram negative pathogens. The methanolic extracts showed better results because polyphenols groups are extracted with polar solvent. The essential oil eo extracts of these allium plants garlic and onions exhibited marked antibacterial activity, with garlic showing the highest inhibition and green onion the lowest. Ginger showed very poor antimicrobial properties against either of the organisms. Antifungal studies in the plant extracts of turmeric. It was concluded that cao nps showed good antimicrobial activity and consequently had improved the shelf life when added to minced meat. It was found that most plant extracts studied had antibacterial and antifungal activities.
Pdf antimicrobial activity of the extract of garlic and onions. However, little research has been undertaken on the antifungal activity of these extracts. The essential oil was extracted by supercritical co 2 extraction in our lab as described by ye and lai 2012. Antimicrobial activity of extracts of red mangle bark, garlic. The various medicinal properties of garlic allium sativum l. Antimicrobial activity of ginger zingiber officinale. A comparison of the antifungal properties of onion allium.
Antimicrobial activity against staphylococcus aureus from methanolic and aqueous extracts of dried a. Antibacterial activity of eo extracts onions and garlic eo extracts exhibited different. Antimicrobial screening of garlic allium sativum extracts. Garlic is a hardy perennial member of onion family. Research article open access antimicrobial activity of onion. The aims of this study were to test the efficacy of essential oil of allium cepa against food spoilage and foodborne pathogenic microorganisms and its antioxidant activity. Onion allium cepa is a bulbous plant widely cultivated in almost every country of. Antimicrobial effect of ginger, garlic, honey, and lemon.
Later studies should similar activity to modern antibiotic including chloramphenicol. Test methodologies were identified as capable of producing. A classroom demonstration of garlic extract and conventional. Introduction garlic is widely used around the world for its pungent flavor as a seasoning or condiment. Chemical composition of the oil was analysed by gcms and the most representative compounds of the essential oil were organosulfurcontaining compounds, such as methyl 5methylfuryl sulfide 18. The essential oil revealed an interesting antimicrobial effect against the tested microorganisms with the mic and mbc values in the ranges of 0. Aqueous extracts of garlic allium sativum and onion allium cepa were tested for activity against grampositive organisms, gramnegative organisms and fungi. We set out to determine if pure allicin and aqueous garlic extracts age exhibit antimicrobial properties against the burkholderia cepacia complex bcc, the major bacterial phytopathogen for alliums and an intrinsically multiresistant and life. Processing of samples first plant juice was extracted for antimicrobial activity. However, dried and autoclaved extracts of shallot showed more activity than similar extracts of oni on and garlic.
Among the onion and garlic, garlic is found to be slightly better species in antimicrobial activity than onion. Effect of eo extracts of onion and garlic on growth of f. Influence of two plant extracts on broilers performance, digestibility, and e. Cancer prevention by organosulfur compounds from garlic and onion. Antibacterial effect of garlic allium sativum against. The antibacterial effect of ginger and garlic extracts on some pathogenic. Antibacterial activity of aqueous garlic allium sativum extracts and virgin coconut oil and their combination against bacillus cereus atcc 14579 and escherichia coli atcc 8939 ninda t. However, little information is available emphasizing the preservative and antimicrobial properties of spices. Garlic extracts have shown antifungal activity when tested in vitro and their use has been suggested in the treatment of oral and vaginal candidiasis. Antimicrobial activity of essential oil extracts of various.
Antimicrobial properties of garlic oil against human enteric. Antibacterial effect of herbs and spices extract on. Benkeblia department of biology, mostaganem university, p. In addition, larger clear zones were observed at higher concentrations against both microorganisms. The screening of antibacterial activity of extracts was carried out using the agar well. H2 garlic and ginger extracts mixed show enhanced anti microbial activity against staphylococcus aureus. Pdf antibacterial activity of garlic extract allium sativum against. Apr 14, 2010 antimicrobial and antioxidant activity of crude onion allium cepa, l. Some years later garlic was shown to have similar effect activity as penicillin.
Apr 27, 2012 in this study heat effect on antimicrobial activity of garlic and ginger was not checked as it is already reported that antimicrobial activity of garlic is affected by heating at 100c for 3060 minutes. On the other hand in our study we have extracted the ginger in soybean which may protect the antimicrobial activity of ginger at high temperature and as a result good antimicrobial activity was found. The antimicrobial activity derived from alliums was identified nearly 70 years ago and subsequently the chemical structure of allicin 2propenylthiosulphinate, figure 1 and its. Antimicrobial effects of calcium oxide nanoparticles and some. Garlic contains a higher concentration of sulfur compounds which are responsible for its medicinal effects. Antimicrobial activity of vinegar and garlic extracts j china condiment. Hernandez f, edward j, garcia v, otrikh j, megias md, 2009. Antimicrobial properties of garlic oil against human. A total amount of 100 grams of cleaned garlic were cut into small pieces by sterile knife and blended with 100 ml bidistilled water using sterile warring blender at. Antimicrobial properties of spices have been documented in ancient literature and the interest continues to the present 1114.
Jan 07, 2016 onion showed good antimicrobial properties against s. In addition, cao nps solution, sage, garlic and onion extracts demonstrated superior antibacterial effects. Garlic peel ethanol extract demonstrated both antioxidant and antibacterial activities in cooked beef. Minimum inhibitory concentrations mic, minimum bactericidal concentrations mbc, and disc and agar well diffusion. Azzouz and bullerman, 1982 which was confirmed in the paired mean comparison of plant extracts on growth of all test fungi compared with pda without plant extracts being. Test methodologies were identified as capable of producing underestimates of go activity. Jorgensen, map faller, rh yolken manual of clinical microbiology 8th eds. Oct 10, 2016 comparatively, garlic extracts inhibited bacterial growths than tetracycline and penicillin. The yellow onion extract caused a reduction of mycelial dry weight of a. Jun 01, 2018 the antimicrobial activity of garlic and onion extracts. H1 ginger and garlic extracts do not show anti microbial activity on staphylococcus aureus. In vitro confirmation of the antimicrobial action of fresh and freezedried garlic extracts against human pathogenic bacteria 2, fungi 3, and viruses 4 supports these applications. The yellow onion extract caused a reduction of mycelial dry weight of. This study was conducted to elucidate minced beef stabilization properties of hydroalcoholic extracts of commonly used culinary spices from pakistan against meat oxidative stress and microbial spoilage.
Abstract antimicrobial activity of different concentrations 50, 100, 200, 300 and 500 mll of essential oil extracts of three type of onions green, yellow and red and garlic against two bacteria, staphylococcus aureus, salmomella enteritidis, and three fungi, aspergillus niger, penicillium cyclopium and fusarium oxysporum, was investigated. Gupta and viswanathan reported that garlic extract stored at room temperature had less activity against b. Therefore, it is recommended to use garlic and ginger in different raw forms like pickle, garlicginger bread, curry powder, sauces, raw. Comparatively, garlic extracts inhibited bacterial growths than tetracycline and penicillin. Antimicrobial activity of ethanolic extracts of syzygium. Sihombing 1, jansen silalahi 1, dwi suryanto 2 1department of pharmaceutical chemistry, faculty of pharmacy, university of sumatera utara. Assessment of antimicrobial activity of onion allium cepa and garlic.
Antibacterial activity of extracts of allium sativum garlic and allium cepa. Hydroalcoholic extracts of six selected spices, namely, onion, ginger, turmeric, coriander, fennel, and mint, were evaluated to inhibit microbial growth in minced beef under. Antimicrobial activity of the extracts was carried out individually and compared considering triplicates of all the extracts. The essential oil eo extracts of these allium plants garlic and onions exhibited marked antibacterial activity, with garlic showing the highest inhibition and. Antimicrobial and antioxidant activities of the essential oil. Heat stable antimicrobial activity of allium ascalonicum. It has been reported that a human would need to eat. Antimicrobialactivityofessentialoilextractsofvariousonions. Discussion the result showed the both clinical and standard isolates of s.
Investigation of antioxidant and antimicrobial properties of. A study of antimicrobial activity of few medicinal herbs. It is concluded that garlic and onion act as an antagonist to the gammaproteobacterial pathogens studied here and if experimented on the synergistic action of both these bulbs active compounds, it might provide good results in future. A significant growth inhibition was shown by most of the organisms, tested at random. Antimicrobial activity of essential oil extracts of various onions. Antimicrobial effects of calcium oxide nanoparticles and. Inhibitory effect of allium sativum and zingiber officinale. Interestingly, comparison of the antimicrobial activity of four independent age preparations showed little batch. Preparation of garlic extracts garlic bulb was washed in tape water. Garlic can provide proper management for bacterial growth ranging from disinfectant, antiseptic, bacteriostatic and even bactericidal characteristics. Pdf antimicrobial activity of the extract of garlic and. The antibacterial effect of ginger and garlic extracts on. Antimicrobial activity of garlic and onion extracts.
Then the garlic and onion pieces were grinded in electric blender separately. Materials and methods plant material garlic bulbs allium sativum linn. Also, garlic powder and onion powder enhanced the shelf life. Antimicrobial activity of extracts of red mangle bark. The inhibitory effect of these spice extracts at varying concentration on listeria monocytogenes is shown in table2. Antimicrobial activity of different concentrations 50, 100, 200, 300 and 500 mll of essential oil extracts of three type of onions green, yellow and red and garlic against two bacteria, staphylococcus aureus, salmomella enteritidis, and three fungi, aspergillus niger, penicillium cyclopium and fusarium oxysporum, was investigated. Fungi were more sensitive to shallot extract than bacteri a. Antimicrobial and antioxidant activities of the essential. Antibacterial actin of vinegar against foodborne pathogenic bacteria including escherichia coli o157.
The yellow onion extract caused a reduction of mycelial. The antimicrobial activity of garlic and onion extracts. The present study was designed to evaluate the in vitro antimicrobial activity of ethanolic extracts of syzygium aromaticum clove and allium sativum garlic against grampositive and gramnegative food associated bacteria bacillus subtilis, b. In vitro antibacterial activity of aquatic garlic extract. Antimicrobial activity of essential oil extracts of. The inoculums were spread evenly over plate with sterile glass spreader.
Introduction allium is a monocot genus of flowering plants, informally referred to. The antimicrobial activities of garlic and other plant alliums are primarily based on allicin, a thiosulphinate present in crushed garlic bulbs. Some years later garlic was shown to have similar effectactivity as penicillin. Luis nunez oreza, betty sarabia alcocer, zelyna kassandra cardenas garcia, giezi espadas granados, paulino tamay segovia, selene blum dominguez, baldemar ake canche, 4francisco aguirre crespo, javier maldonado rivas and rafael mex alvarez. It had the highest level of inhibition on the fungus candida albicans garlic, which showed weak antimicrobial effect on the 62 mm, while the lowest level of antimicrobial activity recorded in bacillus subtilis mm table 1. The test isolates showed variable susceptibility to the garlic and ginger extract aqueous and ethanolic and to other antibiotics cloxacillin, cefepime, cefoxitin, clindamycin and tobramycin. Sterile cotton swab was dipped in to the prepared inoculums and seeded all over the nutrient agar plate by rotating through an angle of 60o. In an attempt to quantitate the in vivo activity of garlic extracts, one research group administered 25. When individual comparison of the extracts was made, garlic showed greatest antimicrobial activity with a mean zone of inhibition 34. The development of new antimicrobial agents against resistant pathogens is increasing interest. The aim of this study is to test the antibacterial effect of various herbs and spices on escherichia coli.
Antimicrobial and antioxidant activities of red onion, garlic and leek. Garlic extract at the higher concentration completely inhibited radial growth of both fungi. Therefore, the methanolic extracts from different parts of four medicinal plants used locally in folk medicine were evaluated for antimicrobial activity. Even the forefather of antibiotic medicine louis pasture acknowledged garlic to be an effective antibiotic. The bioautographic and diffusion methods are known as qualitative techniques since these methods will only give an idea of the presence or absence of substances with antimicrobial activity. Aqueous extracts of garlic allium sativum and onion allium cepa were tested for activity against grampositive. Garlic extract has antimicrobial activity against many genera of bacteria, fungi and viruses. Investigation of antibacterial effects of garlic allium. Both ginger and garlic extracts have bacteriostatic effect on the organism. This project work will be limited only to anti microbial activity of garlic and ginger extracts against staphylococcus. Research article open access antimicrobial activity of. In vitro antibacterial activity and stability of garlic. The activity of the onion extracts was stable even after 48 hours in the culture medium.
Antimicrobial activity of allium cepa against bacteria. At first, skins of the garlic, turmeric, ginger and the onion bulbs were peeled out and washed with sterilized water and air dried for 1 hour and cut in small pieces. Age displayed antimicrobial activity against all of the 38 bcc isolates table 1 with mics ranging from 0. Onion, garlic, human pathogens, antimicrobial activity, solvent extracts. Garlic allium sativum has traditional dietetic and medicinal uses as an antiinfective agent 1.
This research aim at testing the antifungal activity of organic ethanol extracts of onion allium cepa, ginger zingiber officinale and garlic allium sativum against three. Methodologies for estimating the antimicrobial activity of go were assessed and go, go sulfide constituents, and garlic powder gp were compared in tests against human enteric bacteria. Antibacterial activity of garlic extract against human pathogenic. Besides that, garlic may have the ability to prevent and manage viral, fungal and even helminthes infections. Essential oil extracts of onion and garlic can be used as natural antimicrobial additives incorporated in various food products 1617. Antifungal studies in the plant extracts of turmeric, ginger. Phytochemicals present in medicinal plants have health benefits and antimicrobial activity against some pathogenic bacteria. Investigation of antioxidant and antimicrobial properties. Zasshi k, entani e, asai m, tsujihata s, tsukamoto y, ohta m. Garlic extracts activity test well diffusion method screening screening of the antibacterial activity was performed by well diffusion technique saeed et al. Antibacterial activity of aqueous garlic allium sativum. The antimicrobial effects of aqueous garlic extracts are well established but those of garlic oil go are little known. The essential oils exhibited higher effect than ethanolic and aqueous extracts against all tested microorganisms, especially at 60 mgml. Polyphenolrich extracts of traditional culinary spices.
This result suggests that no decomposition or volatility of onion extracts occurred in the culture medium. Some common spices and plants with antimicrobial and. Comparatively, 50 and 100 mll concentrations of onions extracts were less inhibitory than 200, 300 and 500 mll concentrations. Minimum inhibitory concentrations mic, minimum bactericidal concentrations mbc. Antimicrobial activity and thiosulfinates profile of a. The onion, a natural alternative to artificial preservatives.